FAP MASTR

The FAP MASTR amplifies all coding regions of 2 genes, APC and MUTYH, implicated in Familial Adenomatous Polyposis via just 3 multiplex PCR reactions.

Product characteristics:

• > 97% on-target specificity
• 52 amplicons for complete coding exon coverage
• > 30 bp of flanking intron coverage(*)
• Amplicon normalization is achieved through careful multiplex optimization and fixed mixing scheme
• Robust standard PCR protocol 
• Maximum of 50 ng input DNA required per multiplex reaction
• Increased throughput by sequencing more samples in parallel
• Suited for workflow automation
• Cost-effective target amplification, minimizing hands-on-time

The assay is compatible with the Amplicon Sequencing kit of Roche Diagnostics for the GS-FLX and GS Junior platforms. An adapted protocol for "short read sequencing platforms" is also available, you can find these protocols in the section below (downloads).  

We recommend the use of our 454 MID kits to all customers using 454 sequencing technology. These optimized kits contain all reagents for MID labeling and Genescan analysis. Please check the "MASTR related products" group for more details.

(*) Except 3' of APC exon 13: 6bp of flanking intron covered due to presence of an Alu repeat.