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Flagged You-MAQ : your personal design tool for MAQ analysis
Submitted by pronovix_admin on Mon, 07/26/2010 - 10:05.
We developed the flagged You-MAQ assay service to assist you in developing a personal MAQ assay to analyze your CNV region of interest, via our free design interface. Through this interface you access our primer design technology, by entering the human genomic region of interest (min 30 Kb). The system will generate a maximum of 5 primer pairs which you order with your preferred supplier. These multiplex primers are to be used with Multiplicom's flagged You-MAQ control assay kit (YM-0091), containing Taq Polymerase and primers targeting 6 control amplicons on 6 different chromosomes.
A list with the available flagged You-MAQ Control Assays is available in our catalog.
How does flagged You-MAQ work ?
- Step 1 : Log-in at Multiplicom.com and enter the genomic region of interest in the flagged You-MAQ interface
- Step 2 : You receive an e-mail containing the primer sequences for your target amplicons which you can order with your preferred oligo provider
- Step 3 : Order the flagged You-MAQ Control Assays from Multiplicon
- Step 4 : Mix your custom target primers with the flagged You-MAQ control primers, add 20-50 ng of purified sample DNA and run the multiplex PCR. A detailed protocol is available.
- Step 5 : Perform fragment analysis and import the raw data into our MAQ-S software. The comparison of normalized peak areas between the target amplicons and control amplicons results in a dosage quotient, indicating the copy number of the target amplicons.
REMARK : NCBI build 37.1 is used for this interface
Use following link to do the conversion from build 36.1 to build 37.1 : http://genome.cse.ucsc.edu/cgi-bin/hgLiftOver